Publication | Open Access
iLIF: illumination by Laser-Induced Fluorescence for single flash imaging on a nanoseconds timescale
43
Citations
19
References
2011
Year
EngineeringBiomedical EngineeringTransient ImagingLaser CoherenceOptical DiagnosticsBiomedical OpticNanoseconds TimescaleComputational ImagingBioimagingLight MicroscopyBiophysicsNovel Imaging MethodSingle FlashFluorescence ImagingBiophotonicsLaser-induced FluorescenceComputational Optical ImagingOptical ImagingFluorescence MicroscopyBiomedical ImagingBiomedical PhotonicsImagingLight Source
The challenge in visualizing fast microscale fluid motion phenomena is to record high-quality images free of motion-blur. Here, we present an illumination technique based on laser-induced fluorescence which delivers high-intensity light pulses of 7 ns. The light source consists of a Q-switched Nd:YAG laser and a laser dye solution incorporated into a total internal reflection lens, resulting in a uni-directional light beam with a millimeter-sized circular aperture and 3° divergence. The laser coherence, considered undesirable for imaging purposes, is reduced while maintaining a nanoseconds pulse duration. The properties of the illumination by laser-induced fluorescence (iLIF) are quantified, and a comparison is made with other high-intensity pulsed and continuous light sources.
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