Publication | Open Access
DESIGN AND TESTING OF A CALORIMETER FOR MICROBIOLOGICAL USES
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1976
Year
EngineeringDifferential Scanning CalorimetryDesign And TestingAnaerobic DigestionRotatory CalorimeterCalibrationBioenergeticsBioprocess MonitoringCalorimetryMicrobial EcologyEnvironmental MicrobiologyCalorimeter ResponseCalorimetric MethodFood SafetyMicrobial GrowthPhysiologyMicrobiologyMetabolismMedicineQuantitative Microbiology
A rotatory calorimeter of the conduction type was remodelled to fit the continuous study of microbiological processes. Provisions for aeration and agitation made it possible to be used for calorimetric studies of microorganisms aerobically growing in a 5-ml culture. The time constant of the calorimeter response was found to be 8min and the calorimeter could be used for most kinetic studies on microbial cultures.By using a separate culture apparatus, quantitative analyses can be made on samples taken from parallel culture and the results can be correlated with the thermogram obtained with the calorimeter. The performance of the instrument was demonstrated with the aerobic growth of Saccharomyces cerevisiae. Calorimetric study by the use of the present model may give a detailed information at the cellular level about microbial growth processes when combined with turbidity measurements.