Comparison of the Histology, Gene Expression Profile, and Phenotype of Cultured Human Limbal Epithelial Cells from Different Limbal Regions

Abstract

No major transcriptional or phenotypic differences were observed in cultured HLECs derived from different regions of the limbus. However, explants of superior origin demonstrated the highest outgrowth success rate and generated epithelia with greater epithelial thickness and number of cell layers, which may prove useful for transplantation purposes.