FEMS Microbiology Letters · 1986 · 42 citations · 8 references
Life SciencesBiotechnologyMicrobiologyMycologyAntibiotic ProductionFungal BiotechnologyProtoplast CultureCulture-based Isolation Of ActinomycesProtoplast Culture MediaProtoplast IsolationProtoplast PurificationProtoplast RegenerationProtoplast TechnologyProtoplast TransformationProtoplast Viability Assays
A standard procedure for the preparation of viable Streptomyces protoplasts was found to be unsuccessful for the rifamycin-producing actinomycete Nocardia mediterranei. An alternative method for N. mediterranei protoplast preparation is described. This method was also found to be applicable to several other ansamycin-producing actinomycetes.
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Transformation of plasmid DNA into Streptomyces at high frequency
Mervyn J. Bibb, Judith M. Ward, David A. Hopwood · Nature · 1978 · 280 citations