The Influence of Simulated Light Scattering on Automated Perimetric Threshold Measurements

TLDR

The study simulated ocular media scattering by placing six ground‑glass diffusers in five subjects’ right eyes and measured OCTOPUS and Humphrey perimetric thresholds at multiple nasal meridian angles. The simulated scattering produced a 2.7‑ to 16.7‑dB drop in stimulus intensity, reducing differential light sensitivity and contrast sensitivity while leaving visual acuity largely unchanged, indicating that even minimal scattering can bias threshold measurements.

Abstract

• The effect of light scattering by ocular media opacities on OCTOPUS and Humphrey perimeter threshold measurements was simulated with randomly ordered sequences of six ground-glass diffusers in the right eyes of five subjects. Threshold measurements were performed at 0°, 5°, 10°, 15°, 20°, and 25° nasally along the 180° meridian with the F4 program on an OCTOPUS perimeter, and with twice-repeated profiles on a Humphrey perimeter. The reduction in differential light sensitivity correlated well with the 2.7-dB to 16.7-dB reduction in stimulus intensity caused by the 46% to 98% scattering of incident light by the diffusers. Contrast sensitivity in the presence of a glare source (Miller-Nadler glare test) was also affected by the diffusers, such that a 75% contrast target was not visible through the strongest diffuser whereas a 5% contrast target was visible without a diffuser. Conversely, the diffusers had very little effect on visual acuity measurements that were performed with projected high-contrast targets in a darkened room. Our data suggest that even minimal light scattering, such as might be caused by a cataract that has a relatively insignificant effect on visual acuity, may influence threshold measurements.