Simultaneous Measurement of Plasma Progesterone, 17-Hydroxyprogesterone and Estradiol-17B by Radioimmunoassay

Abstract

Abstract Combining a simple chromatographic system on celite microcolumns with partially specific antisera as binding reagents, simultaneous radioimmunoassay of progesterone, 17-hydroxyprogesterone, and estradiol-17B could be performed on the same aliquot of plasma. These steroids have been measured in plasma samples obtained from subjects of both sexes under various conditions.