Retention Behavior of Vitamin D and Related Compounds During High-Performance Liquid Chromatography

Abstract

Abstract The retention behavior of vitamin D2-D5 and provitamin D2-D5 are examined using high-performance liquid chromatography. Inclusion chromatography using cyclodextrin as the mobile phase additive in reversed-phase high-performance liquid chromatography is also used for this purpose. Reversed-phase high-performance liquid chromatography is more effective than normal-phase high-performance liquid chromatography in separating these analogs. The addition of methyl- β - cyclodextrin to the mobile phase is effective in separating the pair of vitamin-D2 and - D3 or provitamin-D2 and -D3 The separation of the pair of stereoisomeric Cookson-type derivatives of vitamin-D2 or -D3 was also examined and found that normal-phase high-performance liquid chromatography is effective for this purpose.