Simultaneous Determination of Norfloxacin and Tinidazole in Tablets by Reverse Phase High Performance Liquid Chromatography (RP - HPLC).

Abstract

Abstract A new simple, precise, rapid and selective HPLC-RP method has been developed for the simultaneous determination of Norfloxacin and Tinidazole in formulations, using 0.2 % Triethylamine (TEA) in water : Acetonitrile (80:20,v/v) and pH adjusted to 2.6 to 2.8 with Phosphoric acid, as a mobile phase, and C18 SHODEX column (5 micron, 25 cm × 3.9 mm, ID) as stationary phase. Detection was carried out using a UV detector at 311 nm Linearity range and percentage recoveries for Norfloxacin and Tinidazole were 20 - 200 μg/mL and 30 - 300 μg/mL, 999.91 % and 99.94 % respectively.