Crayfish plague epizootics in Germany-classification of two German isolates of the crayfish plague fungus Aphanomyces astaci by random amplification of polymorphic DNA

Abstract

Following 2 outbreaks of crayfish plague m southern Germany, the causative agent, the oomycete fungus Aphanomyces astaci, was isolated from the diseased Astacus astacus. The identity of the 2 strains was confirmed using established techniques, such as physiology, spore production and the fact that the isolated strains were hlghly virulent for A. astacus in infection experiments. The relationship between these German strains and other A astaci strains was Investigated using randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The German strains were found to be closely related to a strain that had been isolated from PacLfastacus len~usculus from Lake Tahoe, USA.