High-level secretion of human α1-antitrypsin from Saccharomyces cerevisiae using inulinase signal sequence

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +13

Transformation of intact yeast cells treated with alkali cations

Hisao Ito, Y Fukuda, Kousaku Murata et al. · Journal of Bacteriology · 1983 · 7.7K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +10

Cleavage of structural proteins during the assemble of the head of bacterio-phage T4

Ulrich K. Laemmli · Nature · 1970 · 5.7K citations

Life Sciences, Microbiology, Molecular Biology +13