Modified method for measuring acid phosphatase activities in forest soils with high organic matter content

Abstract

Abstract The acid phosphatase activity (APA) is a key factor in phosphorus (P), mineralization, P nutrition of plants, and P cycling. However, the widely used method of Tabatabai and Bremner for APA determinations is seriously restricted in soils with high organic matter contents, owing to interference in photometric detection by dissolved organic matter. The objectives of this study were: A) to modify the procedure of Tabatabai and Bremner with a view to its application to forest soils rich in organic carbon, b) to determine APA contents in forest soils of the Sierra de Gata mountains, and c) to establish relationships between the APA with different soil parameters. The modified procedure was well adapted to forest soils with high organic matter contents and had good reproducibility, while maintaining the relative simplicity and accuracy of the original Tabatabai and Bremner procedure. APA in the forest soils studied was high but did not correlate with labile P. The APA in the soils studied depended principally on availability of readily degradable energy sources and soil nitrogen (N).