Identification of the allergenic proteins of<i>Ipomoea fistulosa</i>pollen: Partial characterization and sensitivity test

Abstract

Total soluble proteins were extracted from the pollen of Ipomoea fistulosa and subjected to ammonium sulphate precipitation at 80% saturation.Total protein was separated on SDS Polyacrylamide gel.Each band was recovered from the gel by electroelution avoiding the time-consuming and expensive biochemical methods of column chromatography.The allergenic proteins were identified by skin tests.The fourth and the fifth band in the protein profile of I. fistulosa, IF 4 (47 kDa) and IF 5 (42 kDa) were the two major allergenic components of I. fistulosa.The protein profiles showed slight variations in the pollen collected from different geographical regions.