Detection of complement components on unlysed erythrocytes from acid hemolysis and thrombin test reactions in paroxysmal nocturnal hemoglobinuria.

Abstract

paper: PNH, paroxysmal nocturnal hemoglobinuria; PNHE, erythrocytes from patients with PNH; AH- PNHE, residual, unlysed PNH erythrocytes surviving the acid hemolysis reaction; Thr-PNHE, residual, un- lysed PNH erythrocytes surviving thrombin test hemolysis; EAC' hemolysis, in vitro hemolytic reaction in- volving erythrocytes (E), antibody (A), and complement (C') ; C'1, C'2, C'3, and C'4, the first, second, third, and fourth components of complement; fi1m-globulin, hu- man C'4; 8,c-globulin, a subcomponent of human C'3.