FEBS Letters · 2003 · 53 citations · 12 references
Life SciencesMicrobiologyMolecular BiologyMicrobial GeneticsNucleic Acid BiochemistryTranscriptionCore Promoter ElementsPromoter ArchitecturePromoter RecognitionPromoter RegulationRna Polymerase FunctionRna Polymerase StructureAlanine Scanning MutagenesisBacterial PromotersBacterial Rna Polymerase StructureCore Promoter RecognitionEscherichia ColiPromotersRna Polymerase I Promoter RecognitionRna Polymerase I Promoter Regulation
Previous work has shown that the base sequence of the DNA segment immediately upstream of the -10 hexamer at bacterial promoters (the extended -10 element) can make a significant contribution to promoter strength. Guided by recently published structural information, we used alanine scanning and suppression mutagenesis of Region 2.4 and Region 3.0 of the Escherichia coli RNA polymerase sigma(70) subunit to identify amino acid sidechains that play a role in recognition of this element. Our study shows that changes in these regions of the sigma(70) subunit can affect the recognition of different extended -10 element sequences.
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Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 Å resolution
Dmitry G. Vassylyev, Shun‐ichi Sekine, Oleg Laptenko et al. · Nature · 2002 · 752 citations
Structure of the Bacterial RNA Polymerase Promoter Specificity σ Subunit
Elizabeth A. Campbell, Oriana Muzzin, Mark Chlenov et al. · Molecular Cell · 2002 · 505 citations · Full text