Reference intervals for<scp>G</scp>reyhounds and<scp>L</scp>urchers using the<scp>S</scp>ysmex<scp>XT</scp>‐2000i<scp>V</scp>hematology analyzer

Abstract

Background The need for breed‐specific reference intervals ( RIs ) for G reyhounds has been identified. As L urchers are a sighthound cross‐breed, specific RIs may also be needed for these dogs. Hematologic RIs for G reyhounds and L urchers using the S ysmex XT ‐2000i V hematology analyzer have not been established. Objectives The aims of this study were to establish RIs for G reyhounds using the S ysmex XT ‐2000i V , to investigate whether RIs for G reyhound and nonsighthound dogs could be transferred to L urchers, and to establish new RIs for L urchers if transference was not possible. Methods Data were retrieved retrospectively from a database of blood donor dogs. G reyhound RIs were established using nonparametric methods based on a reference population of 179 dogs. For the RI transference study, 38 L urchers were selected, following guidelines proposed by the C linical and L aboratory S tandards I nstitute. When transference was not appropriate, new RIs were generated using the robust method. Results G reyhound RIs for the S ysmex hematology analyzer reflected known differences in this breed with a tendency toward higher RBC mass and lower WBC and platelet counts. RIs for hemoglobin concentration, HCT , MCV , MCH , MCHC , and WBC , neutrophil, lymphocyte, monocyte, and platelet counts for G reyhounds were suitable for transference to L urchers. For RBC and eosinophil counts, new RIs were established. Conclusion Our study suggests that L urchers share many hematologic characteristics with G reyhounds, but had higher reference limits for RBC and eosinophil counts.