Concomitant action of organic and inorganic nanoparticles in wound healing and antibacterial resistance: Chitosan and copper nanoparticles in an ointment as an example

Abstract

The wound-healing properties of organic and inorganic nanoparticles, i.e., chitosan and copper nanoparticles, cointroduced into an ointment preparation, were investigated. When used individually in the ointment, copper oxide nanoparticles at a concentration of 0.002% and a level of oxidation of up to 90% and nanoparticles of chitosan at a concentration of 0.002% prepared from chitosan with a molecular weight of 10 kDa demonstrated the most profound wound-healing effect. The simultaneous introduction of chitosan and copper nanoparticles into the ointment composition led to a synergistic effect. The most pronounced wound healing was observed for complex ointment preparations containing Cu1Ox sample 2 copper nanoparticles at a concentration of 0.002% and chitosan nanoparticles synthesized from low-molecularweight chitosan (Mw 10 kDa) with a degree of deacetylation (DD) of 89% at a concentration of 0.002%. An additive effect was observed for the complex ointment in comparison with ointment preparations based on individual components. Similar antibacterial effects were observed in ointment preparations based on organic and inorganic nanoparticles.