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Screen Printed Cupric-Hexacyanoferrate Modified Carbon Enzyme Electrode for Single-Use Glucose Measurements
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1999
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EngineeringBioelectrochemistryBiochemical SensorsChemical EngineeringBiosensing SystemsBioanalysisSensor FabricationAnalytical ChemistryBioimagingNanosensorBioelectrochemical SystemChemical SensorDispersed Metal-hexacyanoferrate CatalystWearable BiosensorsBiochemistryElectrochemical CellHigh SelectivityElectrochemistryBiomanufacturingBiomedical DiagnosticsSingle-use Glucose MeasurementsBlood Glucose MonitoringElectroanalytical SensorMedicine
The authors develop a disposable glucose biosensor that embeds cupric‑hexacyanoferrate and glucose oxidase into screen‑printable carbon ink to achieve high selectivity. The sensor shows a markedly reduced overvoltage for hydrogen peroxide reduction, stable response at physiological pH, a detection potential around –0.1 V that eliminates anti‑interference membranes, and simplified fabrication, indicating strong promise for thick‑film biosensors.
ABSTRACT A highly selective disposable glucose biosensor based on the dispersion of cupric-hexacyanoferrate and glucose oxidase within a screen-printable carbon ink is described. The dispersed metal-hexacyanoferrate catalyst offers a marked decrease of the overvoltage for the reduction of the enzymatically-liberated hydrogen peroxide, and a stable response at physiological pH. Such efficient catalytic activity allows tuning of the detection potential to a region (around – 0.1V) where interfering reactions are negligible. Such operation eliminates the need for an anti-interference membrane barrier, and along with the one-step dispersion of the enzyme and electrocatalyst, greatly simplifies the sensor fabrication. The high selectivity and simplified fabrication hold great promise for thick-film biosensors for testing glucose and other important substrates of other oxidases.