Determination of doxazosin in its pharmaceutical formulation by high-performance thin-layer chromatography

Abstract

This paper describes a high-performance thin-layer chromatographic method developed for determination of doxazosin in its pharmaceutical formulation. The analysis was performed on aluminum foil-backed silica gel 60 F 254 HPTLC plates with ethyl acetate-methanol, 9 + 1 (v/v) as mobile phase. Detection was performed at λ = 277 nm. Terazosin hydrochloride, a structurally similar molecule was used as internal standard. The developed method was subjected to statistical validation to determine its accuracy and precision. The repeatability was also determined. System suitability tests were performed to verify that the resolution and reproducibility of the chromatography was adequate for the analysis to be done. The determination of doxazosin in tablets is described.