Fluorescence Activated Cell Sorting

TLDR

The system uses laser‑illuminated fluorescent cells in a liquid stream to generate electrical signals that charge droplets, which are then deflected by electrostatic plates into separate containers. The instrument successfully sorts biological cells, yielding large, viable fractions highly enriched for specific functional types.

Abstract

An instrument has been developed for sorting biological cells. The cells are rendered differentially fluorescent and incorporated into a small liquid stream illuminated by a laser beam. The cells pass sequentially through the beam, and fluorescent light from the cells gives rise to electrical signals. The stream is broken into a series of uniform size drops downstream of the laser. The cell signals are used to give appropriate electrostatic charges to drops containing the cells. The drops then pass between two charged plates and are deflected to appropriate containers. The system has proved capable of providing fractions containing large numbers of viable cells highly enriched in a particular functional type.