The Canadian Journal of Chemical Engineering · 1986 · 39 citations · 14 references
Celite ParticlesEngineeringBioelectrochemical ReactorBioprocess EngineeringEnzyme ImmobilizationChemical EngineeringBiomass ConversionBiochemical EngineeringContinuous ProductionMetabolic EngineeringChemical BiotechnologyBiomass UtilizationBiocatalysisAvailable Catalyst SupportBiomanufacturingBiorefinery ProductBiotechnologyCelite SupportImmobilized EnzymeMicrobiologyPenicillium Chrysogenum CellsMedicine
Abstract Celite R‐630, a commercially available catalyst support, was used successfully in the batch and continuous production of Penicillin‐G by mycelial cells of Penicillium chrysogenum. As an extension of previous work, the productivity of a 1.2 L three phase fluidized bed bioreactor using the Celite support was compared directly with the same reactor in which cells had been immobilized inside a larger carrageenan support matrix. With Celite particles, maximum yields and specific reaction rates for continuous reactor runs were 0.11 g Pen‐G (K + )/g lactose and 0.05 mmol Pen‐G/h/g protein respectively. These results were similar to those obtained with carrageenan beads. However, on a reactor volume basis, Celite was five times more productive than carrageenan. Results are discussed in terms of the potential for using Celite in industrial bioreactor systems.
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