Chemical Communications · 2015 · 26 citations · 41 references
EngineeringBiotechnologyCell BiologyBiophysical ChemistryBiosensorsSensorsBiosensor DesignCell-surface LabelingOptical BiosensingOptical BiosensorsPeptide ChemistryPeptidomimetic DesignBiosensing PlatformsBiotin-streptavidin ImmobilizationBiotinylation Of Cell-surface MoleculesCell-based Optical BiosensingCell-based Optical BiosensorsLabelingSialic Acid LabelingSurface Functionalization For Biosensing
We have developed peptidomimetic sialic acid (Sia) biosensors using boronic acid and arginine groups on the peptide backbone. The designed peptides were conjugated to fluorescent streptavidin via biotin enabling the optical labeling of cells. This approach provides unique opportunities to detect Sia composition on the cell surfaces and filopodia.
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