Autocrine Growth Effect of IL-8 and GRO?? on a Human Pancreatic Cancer Cell Line, Capan-1

Abstract

A human pancreatic cancer cell line, Capan-1, secretes the chemokines interleukin-8 (IL-8) and growth-related oncogene alpha (GROα). Capan-1 cells also express the chemokine receptor 2 (CXCR2), which is a Giα-protein coupled receptor. Growth of Capan-1 cells was inhibited when anti-IL-8 or anti-GROα monoclonal antibody was added into the culture medium. Pertussis toxin, which blocks Giα also demonstrated a growth-inhibitory effect on Capan-1 cells. These results indicated that IL-8 and GROα act on Capan-1 cells as growth factors in an autocrine manner through CXCR2.