Quantitative real‐time <scp>PCR</scp> combined with propidium monoazide for the selective quantification of viable periodontal pathogens in an <i>in vitro</i> subgingival biofilm model

Abstract

These results demonstrate the efficiency of PMA for differentiating viable and dead P. gingivalis, A. actinomycetemcomitans and F. nucleatum cells, as well as total bacteria, in an in vitro biofilm model, after being exposed to an antimicrobial agent. Hence, this PMA-qPCR method may be useful for studying the effect of antimicrobial agents aimed at oral biofilms.