Bioscience Biotechnology and Biochemistry · 2009 · 41 citations · 27 references
Veterinary MedicineImmunologyInfectious DiseasesImmunizationVaccine ImmunologyVaccinologyAntibody-mediated ProtectionHumoral ImmunityPoultry VaccinationPoultry VaccinologyVaccine Antigen DiscoveryVeterinary VaccinesEpitope-based Vaccine DesignInfectious Bronchitis VaccinesInfectious Bronchitis VirusPeptide EpitopesPeptide-based ImmunomodulationPoultry Vaccine DevelopmentRecombinant Fusion ProteinsWestern Blot Analysis
Peptide vaccine was found to be an effective and powerful approach to a variety of pathogens. To explore multi-epitope based peptide vaccines against infectious bronchitis virus (IBV), the immunogenic peptides were fused to the 3' terminal of glutathione S transferase gene (GST) and expressed in Escherichia coli. ELISA and Western blot analysis showed that the purified fusion proteins had excellent immune activity with chicken anti-IBV serum. During the vaccination course, the candidate peptide vaccines induced strong humoral and cellular response, and provided up to 80.0% immune protection, while all non-immunized chickens in the negative control group manifested obvious typical symptoms and died after virus challenge. Our finding provides a new way to develop multi-epitope based peptide vaccine against IBV.
27
A simple method of estimating 50 per cent end points
Hr Muench · Medical Entomology and Zoology · 1938 · 8.4K citations
Scott A. Callison, Deborah A. Hilt, Tye O. Boynton et al. · Journal of Virological Methods · 2006 · 378 citations
Veterinary Medicine, Infectious Diseases, Veterinary Microbiology +12