Effects of tryptophan and 5-hydroxytryptophan on the number of monoamine-containing cells in the frog taste buds

Abstract

Effects of serotonin precursors on the metabolism of 5-hydroxytryptamine (5-HT) in dumbbell shaped cells of the frog taste buds were studied using fluorescence microscopy. The injection of DL-tryptophan and 5-hydroxytryptophan (5-HTp) resulted in an increase in the cells' fluorescence intensity and in the number of cells in the taste bud. Pyromycin, in doses not affecting the taste bud structure, prevented the tryptophan-induced increase in the number of fluorescent cells. The participation of specific proteins in the mechanism underlying this phenomenon is suggested.