Peroxisome assembly factor–2, a putative ATPase cloned by functional complementation on a peroxisome–deficient mammalian cell mutant

High-efficiency transformation of mammalian cells by plasmid DNA.

C Chen, Hiroto Okayama · Molecular and Cellular Biology · 1987 · 5.3K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +13

Distantly related sequences in the alpha‐ and beta‐subunits of ATP synthase, myosin, kinases and other ATP‐requiring enzymes and a common nucleotide binding fold.

John E. Walker, M Saraste, M J Runswick et al. · The EMBO Journal · 1982 · 5.3K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +13

SRα Promoter: an Efficient and Versatile Mammalian cDNA Expression System Composed of the Simian Virus 40 Early Promoter and the R-U5 Segment of Human T-Cell Leukemia Virus Type 1 Long Terminal Repeat

Yutaka Takebe, Motoharu Seiki, Jun–ichi Fujisawa et al. · Molecular and Cellular Biology · 1988 · 1.3K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +17

A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.

Hiroto Okayama, P Berg · Molecular and Cellular Biology · 1983 · 935 citations

Life Sciences, Molecular Biology, Molecular Genetics +15

SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat.

Yutaka Takebe, Motoharu Seiki, J Fujisawa et al. · Molecular and Cellular Biology · 1988 · 647 citations

Life Sciences, Genetics, Molecular Biology +16