Steroidal Alkaloids from <i>Veratrum maackii</i><scp>Regel</scp> with Genotoxicity on Brain‐Cell DNA in Mice

Abstract

Abstract Two new steroidal alkaloids, 23‐methoxycyclopamine 3‐ O ‐ β‐ D ‐glucopyranoside ( 1 ) and isoecliptalbine ( 2 ), were isolated from the root and rhizoma of Veratrum maackii Regel , together with five known compounds, i.e. , verussurine ( 3 ), verabenzoamine ( 4 ), verazine ( 5 ), isoverazine ( 6 ), and verazinine ( 7 ). Their structures were established by extensive analysis of spectroscopic data, as well as by comparison with literature data. Compounds 1 – 7 could cause DNA damage in the cerebellum and cerebral cortex of mice in a dose‐dependent manner by using single‐cell gel electrophoresis (comet assay).