Phosphorylation-induced hybridization chain reaction on beads: an ultrasensitive flow cytometric assay for the detection of T4 polynucleotide kinase activity

Abstract

A versatile flow cytometric bead assay (FCBA) has been developed for the ultrasensitive detection of T4 PNK activity by integrating the advantages of the phosphorylation-induced hybridization chain reaction (HCR) for fluorescence signal amplification and flow cytometry for the robust, sensitive and rapid signal readout of the microbeads (MBs).