genesis · 2006 · 153 citations · 19 references
Life SciencesCell BiologyDevelopmental BiologyDevelopmental Cell BiologyGene ExpressionStem Cell BiologyEmbryonic Stem CellsGerm Cell DevelopmentGerm Cell DifferentiationPrimordial Germ Cell DevelopmentReporter Gene ModelsTransgenesis StrategiesCell Differentiation TimingGerm Cell Development Gene ExpressionGerm Cell Developmental TimingGerm Cell Lineage SpecificationPrimordial Germ Cell DifferentiationReporter GenesTransgenic Animal GenerationTransgenic Mice
The relationship between germ cells and pluripotent embryonic stem (ES) cells is of particular interest, together with approaches to generate primordial germ cell (PGCs) from ES cells. A critical requirement in these experiments is the ability to unambiguously detect PGCs with the use of, for example, reporter genes. The currently available transgenic reporters do not show exclusive expression in PGCs at their earliest developmental stages. Here we describe the use of germline-restricted expression of stella, which is currently the best marker gene for PGCs. We generated two stella-GFP reporters and show that both transgenes surpass other reporters in terms of timing and specificity of expression in PGCs. Additionally, we demonstrate the usefulness of stella-GFP during the derivation of PGCs from ES cells.
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Derivation of Oocytes from Mouse Embryonic Stem Cells
Karin Hübner, Guy Fuhrmann, Lane K. Christenson et al. · Science · 2003 · 1.1K citations
Blimp1 is a critical determinant of the germ cell lineage in mice
Yasuhide Ohinata, Bernhard Payer, Dónal O’Carroll et al. · Nature · 2005 · 1K citations