Fusion PCR-targeted tylCV gene deletion of Streptomyces fradiae for producing desmycosin, the direct precursor of tilmicosin

One-step inactivation of chromosomal genes in <i>Escherichia coli</i> K-12 using PCR products

Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences · 2000 · 15K citations · Full text

Studies on transformation of Escherichia coli with plasmids

Douglas Hanahan · Journal of Molecular Biology · 1983 · 11.2K citations

Bacteriology, Biotechnology, Escherichia Coli +4

Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant

Peter Cherepanov, Wilfried Wackernagel · Gene · 1995 · 2K citations

Antimicrobial Resistance Gene, Microbial Genetics, Bacteriology +9

PCR-targeted <i>Streptomyces</i> gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin

Bertolt Gust, Gregory L. Challis, Kay Fowler et al. · Proceedings of the National Academy of Sciences · 2003 · 1.4K citations · Full text

Engineering, Molecular Biology, Secondary Metabolite +17

Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.

Mary Ann Bierman, Robert M. Logan, Kathryn A. O'Brien et al. · Gene · 1992 · 1.4K citations

Conjugal Transfer, Engineering, Microbial Genetics +11