Polorgrgphic Reduction of Delta 4-3-Ketosteroids in Well-Buffered Media

Abstract

The polarographic reduction of progesterone, testosterone, methyltestos ‐terone, testosterone propionate, 17‐hydroxyprogesterone, 11‐deoxycorticosterone, 11‐deoxycorticosterone acetate, and 11‐deoxy‐17‐hydroxycorticosterone has been studied in relation to the changes in the wave effected by pH, temperature, concentration, and mercury pressure. The system used was 50% aqueous alcohol with organic buffers. Testosterone was also examined at pH 7 and above in phosphate and borate buffers. Wave splitting was found in these buffers that was nonexistent at comparable pH values in organic buffers.