Biology of Reproduction · 2001 · 11 citations · 36 references
Life SciencesGeneticsMolecular BiologyGene ExpressionSignal TransductionTranscriptional RegulationChromatinCis-regulatory ElementsGene Regulatory ElementsNotch SignalingPlacental BiologyPromoter ArchitecturePromoter Region IdentificationRbpj-mediated TranscriptionReporter Gene AssaysTranscription Factor Binding SitesTransient ExpressionTransient Expression SystemsTransient Gene ExpressionYeast Two-hybrid Screening
The rat pregnancy-specific glycoprotein gene rnCGM3 is primarily expressed in the placenta. Previously, three DNase I footprinting sites (FPI, FPII, and FPIII) were identified in the rnCGM3 promoter region, a yeast one-hybrid screen was performed to identify the nuclear factors binding to the FPIII (5'-GCCTGGGAAAAAACTC-3') element, and RBPJ kappa, a downstream effector of the Notch signaling pathway, was identified as one of the FPIII-binding factors. In the present study, the NF kappa B member p65 was identified as another FPIII-binding factor. Electrophoretic mobility shift assays showed that NF kappa B members, including p50 and p65, bound to the FPIII site. The core binding sequence in the FPIII element for p50 and p65 is GGGAAA, which overlaps with that for RBPJ kappa. Competition exists between p50 and RBPJ kappa for binding to the FPIII element. Transient expression analyses revealed that p65 significantly stimulated the expression of a reporter gene directed by the NF kappa B core sequence in the FPIII element. However, RBPJ kappa could block this stimulation. These results suggest that the regulation of rnCGM3 expression involves both NF kappa B and RBPJ kappa, and they are mutually exclusive in the FPIII element.
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Multiple nuclear factors interact with the immunoglobulin enhancer sequences
Ranjan Sen, David Baltimore · Cell · 1986 · 2.8K citations · Full text
Taiji Matsusaka, Kazuo Fujikawa, Yukihiro Nishio et al. · Proceedings of the National Academy of Sciences · 1993 · 963 citations