Cloning and expression analysis of rainbow trout Oncorhynchus mykiss interferon regulatory factor 1 and 2 (IRF-1 and IRF-2)

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

Analytical Biochemistry · 1987 · 63.2K citations

Nucleic Acid Chemistry, Engineering, Natural Sciences +7

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

Piotr Chomczyński, Nicoletta Sacchi · Analytical Biochemistry · 1987 · 46.8K citations

Engineering, Natural Sciences, Rna Isolation +7

‘Touchdown’ PCR to circumvent spurious priming during gene amplification

R.H. Don, Peter Cox, Brandon J. Wainwright et al. · Nucleic Acids Research · 1991 · 2.6K citations · Full text

Polymerase Chain Reaction, Transcriptional Regulation, Gene Amplification +14

Regulated expression of a gene encoding a nuclear factor, IRF-1, that specifically binds to IFN-β gene regulatory elements

Masaaki Miyamoto, Takashi Fujita, Yoko Kimura et al. · Cell · 1988 · 995 citations

Transcriptional Regulation, Nuclear Factor, Signal Transduction +10

Structurally similar but functionally distinct factors, IRF-1 and IRF-2, bind to the same regulatory elements of IFN and IFN-inducible genes

Hisashi Harada, Takashi Fujita, Masaaki Miyamoto et al. · Cell · 1989 · 981 citations

Transcriptional Regulation, Natural Sciences, Genetics +14