Hoehnea · 2015 · 12 citations · 12 references
BiologyMorphological EvidenceLamina LeafPiper LepturumvarPhylogeneticsBotanyPlant AnatomyNatural SciencesPiper Lepturum VarEvolutionary BiologyPiper LepturumMorphologyComparative AnatomyPhytogeographyPlant TaxonomyPlant PhysiologyPlant Histology
This study showed anatomical differences related to Piper lepturumvar. lepturum and P. lepturum var. angustifolium species, sometimes considered varieties and in other cases synonyms. For histological analysis, fully expanded leaves were collected and for analysis by scanning electron microscope (SEM), fragments from the midrib were fixed on both leaf surfaces. The two species revealed differences in plant anatomy and it was observed that the stem of P. lepturum var. lepturum showed persistent wings and papillary epidermal cells, and these characters are absent in P. lepturum var. angustifolium. There was also the presence of raphides only in the lamina leaf and petiole of P. lepturum var. angustifolium, differentiating the two species. Recent studies have shown the importance of plant anatomy with species where there are issues related to taxonomic delimitation.
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Polychromatic staining of plant cell walls by toluidine blue O
T. P. O'Brien, N. Feder, M. E. McCully · PROTOPLASMA · 1964 · 2.3K citations
Flowering Plants of the Neotropics
Margaret B. Gargiullo, Nathan Smith, Scott A. Mori et al. · The Journal of the Torrey Botanical Society · 2004 · 292 citations