Experimental Biology and Medicine · 1966 · 241 citations · 0 references
Lipid AnalysisImmunohematologyLipid PeroxidationImmunologyPlasma 1Analytical UltracentrifugationChemical BiologyOxidative StressProtein PurificationBioanalysisImmunochemistryAnalytical ChemistryHuman SerumChromatographyBiochemistryDensity Gradient SedimentationChromatographic AnalysisPharmacologySummarythe Colloidal NatureIndocyanine GreenNatural SciencesLipoprotein MetabolismLipid ChemistryMedicine
SummaryThe colloidal nature of ICG in aqueous solutions has been characterized by dialysis, ultranltration and spectral absorption measurements. Using gel filtration (Sephadex G-100 and G-200), ultracentrifu-gal flotation, density gradient sedimentation, immuno- and paper-electrophoretic methods, evidence has been accumulated to show that 90% of the ICG in canine serum and 80% of the dye in human serum is associated with globulins. Comparable values for BSP are 60% and 30%. The data suggest that the α1 group of lipoproteins has a higher affinity for the dyes than albumin.