CHARACTERIZATION OF A CHONDROITIN SULPHATE PROTEOGLYCAN FROM OVINE BRAIN

Abstract

Abstract— A proteoglycan has been isolated from ovine brain tissue using a dissociative method of extraction. The preparation was then purified using ion‐exchange and gel chromatography, and an apparent molecular weight of 2 ± 10 6 was estimated. Chemical analysis together with β‐elimination studies strongly suggested that the macromolecule contains chondroitin sulphate chains covalently bound to a common protein core through a serine‐xylose linkage. Immunological studies showed that a common antigenic component exists between the respective protein moieties of the proteoglycan and a proteoglycan isolated from bovine nasal cartilage.