Site specific photo-crosslinking of single stranded oligonucleotides by a complementary sequence equipped with an internal photoactive probe

Abstract

The photo-crosslinking behaviour of oligonucleotide constructs, incorporating photoactive residues 1–5 at a defined position, has been examined in the presence of their DNA (14-mer) and RNA (15-mer) complementary targets using a two-fold excess; it revealed a moderate but promising irreversible binding efficiency, the results of which could be anticipated by molecular mechanics in the case of 4.