ACTIVE GLUCOSE UPTAKE BY STRIPS OF GUINEA PIG INTESTINE; COMPETITIVE INHIBITION BY PHLORHIZIN AND PHLORETIN

Abstract

The active uptake of glucose has been demonstrated using the modified form of the intact-strip method in the golden hamster and guinea pig intestine. Accumulation of glucose by guinea pig intestine reaches a steady-state level within 90 minutes at 37 °C. Prolonged starvation does not appear to enhance active glucose uptake, and in unfasted guinea pigs an endogenous mean value of 2.15 mM glucose is present.A maximum capacity to accumulate glucose occurs at a point approximately 25 inches from the pyloric end of the jejunum.Study of the inhibition of glucose uptake by phlorhizin and phloretin indicates that these compounds inhibit the uptake of glucose competitively.