Doxycycline‐ and tetracycline‐regulated transcriptional silencer enhance the expression level and transactivating performance of rtTA

Abstract

Taken together, our data establish an unappreciated role of doxycycline and tTS in tetracycline-regulated gene expression and the functionality of rtTA, and should shed light on the design of gene therapy vectors based on tetracycline-controlled transcriptional regulation systems.