Journal of Nanoscience and Nanotechnology · 2008 · 10 citations · 0 references
EngineeringBiotechnologyMaterials ScienceBiosensorsElectrochemistrySensorsElectrochemical BiosensorsElectrochemical DetectionElectrochemical SensorsElectrode MaterialsEnzymatic BiosensorsEnzyme-based BiosensorsElectrochemical Enzyme BiosensorsElectrochemical Enzyme SensorsElectrochemical ReductionEntrapmentGold ElectrodesHydrogen Peroxide BiosensorsNanomaterial-modified ElectrodesPeroxidase Enzymes
Enzyme immobilized electrode was fabricated by two methods. In one of the methods, gold-nanoparticles (Au-NPs) prepared by gamma-irradiation were loaded into the copolymer film and horseradish peroxidase (HRP) was immobilized into the Au-NPs loaded copolymer film through physical entrapment. In the other method, the Au-NPs was prepared by electrochemical reduction of Au ions on the surface of poly(Th-AP-TAA) and HRP was immobilized into the Au-NPs. The enzyme immobilized electrodes were tested for electrocatalytic activities towards sensing of H2O2.