Newcastle disease virus f glycoprotein expressed from a recombinant vaccinia virus vector protects chickens against live‐virus challenge

Abstract

Chickens were immunised using a vaccinia recombinant virus (vaccinia-Italien-F), expressing the F protein of Newcastle disease virus (NDV). Immunisation was successful using either TK" cells infected with the vaccinia-Italien-F virus, the recombinant virus grown in TK7 cells and inoculated intracerebrally in one-day-old chickens or the recombinant virus given by wing-web to adult chickens after adaptation by alternate passage in chick embryo fibroblasts and chickens. The use of recombinant viruses expressing the F protein of NDV as vaccines would allow joint application of vaccination and eradication programmes for NDV. Therefore, recombinant viruses obtained in chickens virus vectors are needed.