<title>Experimental set-up and Monte-Carlo model for the determination of optical tissue properties in the wavelength range 330 to 1100 nm</title>

Abstract

The optical properties of different types of tissue were measured in the wavelength range 330 - 1100 nm. The measurements were carried out in native as well as in coagulated tissues. We used the double integrating sphere technique to provide reflection and transmission measurements and a special homogenizing technique to prepare the tissue. The optical properties were evaluated using an inverse Monte-Carlo simulation, considering the geometry of the experimental set-up. All tissues show characteristic absorption bands at 420 nm and 550 nm, related to the strong absorption of hemoglobin. After coagulation the scattering increases drastically while absorption remains nearly unchanged. The anisotropy factor g increases with increasing wavelength and drops down slightly after coagulation.