Gold Electrode Modification with Thiolated Hapten for the Design of Amperometric and Piezoelectric Immunosensors

Abstract

Self-assembly of a hapten monolayer is used for the development of amperometric and piezoelectric immunosensors. A disulfide compound carrying a terminal pyrene group was newly synthesized for the modification of a gold surface. The self-assembled organic monolayer was formed spontaneously through the use of a disulfide head group. The resulting monolayer and its long-term stability on a gold surface were measured by cyclic voltammetry. The application of the hapten monolayer to amperometric and piezoelectric immunosensing of monoclonal antibodies and benazo[a]pyrene (BaP) was also investigated.