A DNA fragment homologous to F₁‐ATPase β subunit was amplified from genomic DNA of <i>Methanosarcina barkeri</i> Indication of an archaebacterial F‐type ATPase

Abstract

A 490 bp DNA fragment was amplified from Methanosarcina barkeri genomic DNA by the polymerase chain reaction (PCR) using oligonucleotide primers designed based on conserved amino acid sequences of the F1-ATPase beta subunits. The amino acid sequence deduced from the DNA sequence of this fragment was highly homologous to a portion of the F1-ATPase beta subunit. This indicates that this archaebacterium has a gene of F-type ATPase in addition to a gene of V-type ATPase.