Proton Magnetic Resonance Studies of 2′-Deoxythymidine, its 3′- and 5′-Monophosphates and 2′-Deoxythymidylyl-(3′, 5′)-2′ -deoxythymidine in Aqueous Solution

Abstract

Proton magnetic resonance studies of 2′-deoxythymidine, its 3′ and 5′-monophosphates and the dideoxynucleoside monophosphate, 2′-deoxythymidylyl-(3′,5′)-2′-deoxythymidine are described. Assignment of the spectral bands are carried out and the derived chemical shift and coupling constant data are discussed in terms of the conformational properties of these molecules. The main conclusion reached is that 3′- and 5′-phosphorylation as well as incorporation into the dinucleoside monophosphate at either the 3′- or 5′-terminus has only slight effects on the conformation of thymidine under the experimental conditions employed.