Publication | Closed Access
Ultrafast Yb:KGd(WO 4 ) 2 laser for multimodal biomedical imaging with reduced photodamage
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Citations
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References
2008
Year
EngineeringLaser ScienceLaser ApplicationsBiomedical EngineeringBioimagingPhotosynthesisMultimodal Biomedical ImagingBiophysicsUltrafast LasersBiochemistryUltrafast YbLaser MicroscopyLaser DevelopmentFluorescence ImagingBiophotonicsOptical ImagingLaser PhotochemistryBiomedical ImagingBiomedical PhotonicsFemtosecond YbUltrafast OpticsLaser-surface InteractionsWo 4
We report on the development and successful application of a femtosecond Yb:KGd(WO<sub>4</sub>)<sub>2</sub> laser for multimodal imaging of various biological samples. Its operation at longer wavelengths, 1029 nm, provided efficient excitation and greatly reduced sample photobleaching. The laser produced ~300 fs pulses with up to 100 nJ of energy at 14.3 MHz repetition rate. This laser system enabled continuous imaging of various live samples for prolonged periods of time. The details of laser development and fluorescence imaging of isolated chloroplasts are reported.
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