NMR spectroscopy in the study of carbohydrates: Characterizing the structural complexity

TLDR

Carbohydrate NMR spectra are difficult to interpret because of structural diversity and limited chemical shift dispersion. The paper introduces guidelines for interpreting carbohydrate 1‑ and 2‑D NMR spectra, taking into account tautomeric, configurational, and conformational equilibria as well as biosynthetic diversity. The authors illustrate how structural features influence chemical shifts and coupling constants in homo‑ and heteronuclear 2‑D NMR spectra and discuss brief applications to carbohydrate metabolism studies. © 2003 Wiley Periodicals, Inc., Concepts Magn Reson 19A: 1–19.

Abstract

Abstract The combination of structural diversity at several levels and limited chemical shift dispersion ensures that NMR spectra of carbohydrates are relatively difficult to interpret. This introduction to applications of NMR spectroscopy for the study of carbohydrates provides guidelines for interpretation of their 1‐ and 2‐D spectra against a background of their tautomeric, configurational, and conformational equilibria in solution and consideration of their biosynthetic diversity. The influence of structural features on chemical shifts and coupling constants is illustrated by the consequences for both homo‐ and heteronuclear 2‐D NMR spectra. Some applications of NMR spectroscopy for studies of carbohydrate metabolism are briefly considered. © 2003 Wiley Periodicals, Inc. Concepts Magn Reson 19A: 1–19, 2003.