FEMS Microbiology Letters · 1992 · 28 citations · 10 references
Life SciencesGeneticsMicrobiologyAntimicrobial ResistanceMedical MicrobiologyMicrobial GeneticsAntibacterial ResistanceAntibiotic ResistanceEfflux-mediated ResistancePlasmid GeneticsPlasmid-mediated ResistanceResistance GenesAntibiotic Resistance GenesAntibiotic Resistance PlasmidsChromosomally Encoded Resistance DeterminantsPlasmid-borne Antibiotic ResistancePlasmid-borne ResistancePlasmid-mediated Resistance TransferResistance Mechanisms In StaphylococciResistance Plasmid Transfer
A gene encoding high-level resistance to ethidium bromide (EB) and antiseptics was isolated from a transferable plasmid, pTZ22, in Staphylococcus aureus. DNA sequencing revealed that the plasmid has two copies of the ebr gene that normally mediates low-level resistance to EB and antiseptics. The efflux rate for EB of strains with duplicated ebr genes was twice the rate of strains with a single ebr gene. It was concluded that the duplication of ebr is responsible for the high-level resistance to EB and antiseptics.
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Duncan A. Rouch, David S. Cram, D. Di Berardino et al. · Molecular Microbiology · 1990 · 301 citations