ACS Applied Materials & Interfaces · 2014 · 46 citations · 32 references
Life SciencesBiochemistryCell BiologyApoptosisMitochondrial BiologyMolecular ImagingApoptosis ImagingFluorescence ImagingFluorescence MicroscopyMitochondrial FunctionMitochondrial MetabolismMitochondrial PhysiologyEnergy Metabolism RegulationFluorescence Imaging ProbesFluorescent Chemical ProbesFluorescent DyesFluorescent ProbesLive-cell Imaging ProbesMitochondrial VisualizationNatural Dyes
Mitochondria, vital organelles existing in almost all eukaryotic cells, play a crucial role in energy metabolism and apoptosis of aerobic organisms. In this work, we report two new flavone-based fluorescent probes, MC-Mito1 and MC-Mito2, for monitoring mitochondria in living cells. These two probes exhibit remarkably low toxicity, good cell permeability, and high specificity; these probes complement the existing library of mitochondrial imaging agents. The new dyes give nearly no background fluorescence, and their application does not require tedious postwashing after cell staining. The appreciable tolerance of MC-Mito2 encourages a broader range of biological applications for understanding the cell degeneration and apoptosis mechanism.
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