Streptococcal Reporter Gene-Fusion Vector for Identification of in Vivo Expressed Genes

Basic local alignment search tool

Stephen F. Altschul, Warren Gish, Webb Miller et al. · Journal of Molecular Biology · 1990 · 92.8K citations

Bioinformatics, Engineering, Information Retrieval +10

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

Detection of specific sequences among DNA fragments separated by gel electrophoresis

E.M. Southern · Journal of Molecular Biology · 1975 · 32.8K citations

Chromatin, Dna Sequencing, Dna +15

One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution.

Cathie T. Chung, Sanna‐Mari Niemelä, R.H. Miller · Proceedings of the National Academy of Sciences · 1989 · 1.6K citations · Full text

One-step Preparation, Engineering, Bacteriology +16

Selection of Bacterial Virulence Genes That Are Specifically Induced in Host Tissues

Michael J. Mahan, James M. Slauch, John J. Mekalanos · Science · 1993 · 662 citations